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gfap  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc gfap
    Gfap, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+gfap/GFAP+XP+Rabbit+mAb/pmc12996796-121-17-18
    Average 96 stars, based on 515 article reviews
    gfap - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Spinal Microglial TLR7 Activation Drives Hyperalgesia in a Lupus Mouse Model via Upregulation of IL-1β, IL-18, and Cav2.2 and Enhanced Glutamatergic Synaptic Activity
    Article Snippet: .. Proteins were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), which were then blocked with 5% nonfat milk in TBST and incubated overnight at 4 °C with the following primary antibodies: rabbit anti-Cav2.2 (1:200; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Iba1 (1:200; Millipore, Burlington, MA, USA), rabbit anti-GFAP (1:200; Cell Signaling Technology), rabbit anti-cathepsin B (1:200; Cell Signaling Technology), rabbit anti-IL-1β (1:200; Proteintech, Rosemont, IL, USA), rabbit anti-IL-18 (1:200; Bioss, Woburn, MA, USA), rabbit anti-MyD88 (1:200; Bioss), rabbit anti-phospho-ERK (1:200; Cell Signaling Technology), rabbit anti-phospho-p38 MAPK (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-c-Fos (1:200; Cell Signaling Technology), rabbit anti-TLR7 (1:200; Bioss), or rabbit anti-GAPDH (1:200; GeneTex, Irvine, CA, USA) as a loading control. .. The blots were then incubated for 2 h at room temperature with the appropriate secondary antibodies: IRDye ® 680LT goat anti-mouse IgG1 (1:5000; LI-COR Biosciences, Lincoln, Nebraska, USA) or IRDye ® 680RD donkey anti-rabbit IgG (1:5000; LI-COR Biosciences), diluted in TBST.

    Article Title: Spinal Microglial TLR7 Activation Drives Hyperalgesia in a Lupus Mouse Model via Upregulation of IL-1β, IL-18, and Cav2.2 and Enhanced Glutamatergic Synaptic Activity.
    Article Snippet: .. Proteins were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), which were then blocked with 5% nonfat milk in TBST and incubated overnight at 4 ◦C with the following primary antibodies: rabbit anti-Cav2.2 (1:200; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Iba1 (1:200; Millipore, Burlington, MA, USA), rabbit anti-GFAP (1:200; Cell Signaling Technology), rabbit anti-cathepsin B (1:200; Cell Signaling Technology), rabbit anti-IL-1β (1:200; Proteintech, Rosemont, IL, USA), rabbit anti-IL-18 (1:200; Bioss, Woburn, MA, USA), rabbit anti-MyD88 (1:200; Bioss), rabbit anti-phosphoERK (1:200; Cell Signaling Technology), rabbit anti-phospho-p38 MAPK (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-c-Fos (1:200; Cell Signaling Technology), rabbit anti-TLR7 (1:200; Bioss), or rabbit anti-GAPDH (1:200; GeneTex, Irvine, CA, USA) as a loading control. .. The blots were then incubated for 2 h at room temperature with the appropriate secondary antibodies: IRDye® 680LT goat anti-mouse IgG1 (1:5000; LI-COR Biosciences, Lincoln, Nebraska, USA) or IRDye® 680RD donkey anti-rabbit IgG (1:5000; LI-COR Biosciences), diluted in TBST.

    Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors.
    Article Snippet: .. Frozen sections were blocked in 5% donkey serum or goat serum in PBS containing 0.3% Triton X-100 at room temperature for 1 h. Thereafter, brain sections were incubated at 4 °C overnight with the following primary antibodies: mouse anti-P2X7 (1:100, #sc-514962, Santa Cruz, USA), rabbit anti-p-STING (1:100, #62,912, CST, USA), rabbit antiIBA1 (1:200, #17,198, CST, USA), goat anti-IBA1 (1:200, #ab5076, Abcam, UK), rabbit anti-GFAP (1:200, #12,389, CST, USA), mouse anti-GFAP (1:200, #3679, CST, USA), rabbit anti-NeuN (1:400, #ab190565, Abcam, UK), mouse anti-NeuN (1:400, #ab104224, Abcam, UK). .. The sections were then washed in PBS and incubated at room temperature for 1 h with the following secondary antibodies: Alexa594-conjugated donkey anti-rabbit IgG, Alexa-488-conjugated donkey anti-mouse IgG, Alexa-594-conjugated donkey anti-mouse IgG, Alexa-488-conjugated donkey anti-rabbit IgG (1:500; Invitrogen, USA).

    Control:

    Article Title: Spinal Microglial TLR7 Activation Drives Hyperalgesia in a Lupus Mouse Model via Upregulation of IL-1β, IL-18, and Cav2.2 and Enhanced Glutamatergic Synaptic Activity
    Article Snippet: .. Proteins were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), which were then blocked with 5% nonfat milk in TBST and incubated overnight at 4 °C with the following primary antibodies: rabbit anti-Cav2.2 (1:200; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Iba1 (1:200; Millipore, Burlington, MA, USA), rabbit anti-GFAP (1:200; Cell Signaling Technology), rabbit anti-cathepsin B (1:200; Cell Signaling Technology), rabbit anti-IL-1β (1:200; Proteintech, Rosemont, IL, USA), rabbit anti-IL-18 (1:200; Bioss, Woburn, MA, USA), rabbit anti-MyD88 (1:200; Bioss), rabbit anti-phospho-ERK (1:200; Cell Signaling Technology), rabbit anti-phospho-p38 MAPK (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-c-Fos (1:200; Cell Signaling Technology), rabbit anti-TLR7 (1:200; Bioss), or rabbit anti-GAPDH (1:200; GeneTex, Irvine, CA, USA) as a loading control. .. The blots were then incubated for 2 h at room temperature with the appropriate secondary antibodies: IRDye ® 680LT goat anti-mouse IgG1 (1:5000; LI-COR Biosciences, Lincoln, Nebraska, USA) or IRDye ® 680RD donkey anti-rabbit IgG (1:5000; LI-COR Biosciences), diluted in TBST.

    Article Title: Spinal Microglial TLR7 Activation Drives Hyperalgesia in a Lupus Mouse Model via Upregulation of IL-1β, IL-18, and Cav2.2 and Enhanced Glutamatergic Synaptic Activity.
    Article Snippet: .. Proteins were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), which were then blocked with 5% nonfat milk in TBST and incubated overnight at 4 ◦C with the following primary antibodies: rabbit anti-Cav2.2 (1:200; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Iba1 (1:200; Millipore, Burlington, MA, USA), rabbit anti-GFAP (1:200; Cell Signaling Technology), rabbit anti-cathepsin B (1:200; Cell Signaling Technology), rabbit anti-IL-1β (1:200; Proteintech, Rosemont, IL, USA), rabbit anti-IL-18 (1:200; Bioss, Woburn, MA, USA), rabbit anti-MyD88 (1:200; Bioss), rabbit anti-phosphoERK (1:200; Cell Signaling Technology), rabbit anti-phospho-p38 MAPK (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-c-Fos (1:200; Cell Signaling Technology), rabbit anti-TLR7 (1:200; Bioss), or rabbit anti-GAPDH (1:200; GeneTex, Irvine, CA, USA) as a loading control. .. The blots were then incubated for 2 h at room temperature with the appropriate secondary antibodies: IRDye® 680LT goat anti-mouse IgG1 (1:5000; LI-COR Biosciences, Lincoln, Nebraska, USA) or IRDye® 680RD donkey anti-rabbit IgG (1:5000; LI-COR Biosciences), diluted in TBST.

    Staining:

    Article Title: Ferroptosis is a novel pathogenic mechanism of FDXR-related disease via disruption of the NRF2 pathway
    Article Snippet: .. For GFAP staining, sections were stained in the CCHMC pathology core with rabbit anti-GFAP (Cell Signaling Technology, #12389) at 1:1000 dilution. .. For Fluoro-Jade C staining, staining was performed using the “Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons” (Biosensis), according to the manufacturer’s instructions.

    Article Title: Ferroptosis is a novel pathogenic mechanism of FDXR-related disease via disruption of the NRF2 pathway.
    Article Snippet: .. For GFAP staining, sections were stained in the CCHMC pathology core with rabbit anti-GFAP (Cell Signaling Technology, #12389) at 1:1000 dilution. .. For Fluoro-Jade C staining, staining was performed using the “Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons” (Biosensis), according to the manufacturer’s instructions.

    Immunolabeling:

    Article Title: Astrocytic GABA controls fidelity of temporal cortical processing in Fragile X Syndrome.
    Article Snippet: .. Cells were blocked for 1 h at room temperature with 5 % NDS in 0.1 % Triton X-100 in 0.1 M PBS and then immunolabeled with mouse anti-S100b antibody (1:500, Sigma-Aldrich, Cat# S2532, RRID: AB_477499) and rabbit anti-GFAP (1:500; Cell Signaling, catalog #12389, RRID:AB_2631098) antibody in blocking buffer overnight at 4 ◦C. .. Coverslips then were washed 3 × 10 min with 1 % NDS in 0.1 M PBS at room temperature followed by the incubation with donkey antimouse AlexaFluor 488 (4 μg/ml, Invitrogen, catalog # A-21202, RRID: AB_141607) and donkey anti-rabbit Alexa Fluor 594 (4 μg/ml, Invitrogen, catalog #A-21207, RRID:AB_141637) for 2 h at room temperature.

    Blocking Assay:

    Article Title: Astrocytic GABA controls fidelity of temporal cortical processing in Fragile X Syndrome.
    Article Snippet: .. Cells were blocked for 1 h at room temperature with 5 % NDS in 0.1 % Triton X-100 in 0.1 M PBS and then immunolabeled with mouse anti-S100b antibody (1:500, Sigma-Aldrich, Cat# S2532, RRID: AB_477499) and rabbit anti-GFAP (1:500; Cell Signaling, catalog #12389, RRID:AB_2631098) antibody in blocking buffer overnight at 4 ◦C. .. Coverslips then were washed 3 × 10 min with 1 % NDS in 0.1 M PBS at room temperature followed by the incubation with donkey antimouse AlexaFluor 488 (4 μg/ml, Invitrogen, catalog # A-21202, RRID: AB_141607) and donkey anti-rabbit Alexa Fluor 594 (4 μg/ml, Invitrogen, catalog #A-21207, RRID:AB_141637) for 2 h at room temperature.



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